大鼠垂体促性腺激素在等电聚焦上的异质性；性别差异及去势后的差异

Heterogeneity of rat pituitary gonadotropins on electrofocusing; differences between sexes and after castration.

作者信息

Robertson D M, Foulds L M, Ellis S

出版信息

Endocrinology. 1982 Aug;111(2):385-91. doi: 10.1210/endo-111-2-385.

DOI:10.1210/endo-111-2-385

Abstract

Anterior pituitary extracts from intact and 4 week postcastration male and female rats were electrofocused in sucrose density gradients within the pH range 3.5-10. Column fractions were combined to cover this pH range in 0.5 pH units and assayed for LH by in vitro bioassay and RIA and for FSH by radioreceptor assay and RIA. The pH distribution of bioactive LH was altered after castration in both sexes, with the proportion of recovered activity in the alkaline pH range increasing (P less than 0.01) from 52-57% in the intact animal to 71-73% after castration. In addition, significantly more bioactivity was recovered in the pH range 7-9.5 with the male (37%) than with either of the female (diestrous, 30% or proestrous, 28%) groups (P less than 0.05). FSH receptor binding activity was located in the pH region 3.5-6.5. Significantly less receptor binding activity was recovered in the pH range 3.5-4.5 with the female groups (39% and 37% diestrous and proestrous, respectively) than the male group (61%; P less than 0.05, P less than 0.01). The distribution of immunoreactive LH and FSH was similar to that observed with the LH in vitro bioassay and FSH radioreceptor assay. It is concluded that the charge distribution of pituitary gonadotropins is altered according to the sex of the animal and after castration. These findings provide further evidence that the type of gonadotropin produced by the pituitary is under endocrine control.

摘要

从完整的以及阉割后4周的雄性和雌性大鼠中提取垂体前叶提取物，在pH值3.5 - 10范围内的蔗糖密度梯度中进行等电聚焦。将柱馏分合并以覆盖该pH范围，间隔为0.5个pH单位，并通过体外生物测定法和放射免疫分析法测定促黄体生成素（LH），通过放射受体分析法和放射免疫分析法测定促卵泡生成素（FSH）。阉割后，两性中生物活性LH的pH分布均发生改变，碱性pH范围内回收活性的比例增加（P < 0.01），从完整动物的52 - 57%增加到阉割后的71 - 73%。此外，在pH值7 - 9.5范围内，雄性（37%）回收的生物活性明显高于雌性组（动情间期，30%；动情前期，28%）中的任何一组（P < 0.05）。FSH受体结合活性位于pH值3.5 - 6.5区域。在pH值3.5 - 4.5范围内，雌性组（动情间期和动情前期分别为39%和37%）回收的受体结合活性明显低于雄性组（61%；P < 0.05，P < 0.01）。免疫反应性LH和FSH的分布与LH体外生物测定法和FSH放射受体分析法中观察到的分布相似。结论是，垂体促性腺激素的电荷分布根据动物的性别和阉割后情况而改变。这些发现进一步证明垂体产生的促性腺激素类型受内分泌控制。