Linnainmaa K, Wolff S
Environ Mutagen. 1982;4(3):239-47. doi: 10.1002/em.2860040306.
To see if DNA crosslinks are involved in the induction of sister chromatid exchange (SCE), Chinese hamster ovary cells were exposed to two bifunctional alkylating agents, mitomycin C and 8-methoxypsoralen, and their monofunctional derivatives, decarbamoyl mitomycin C and angelicin. The data indicate that monoadducts, rather than crosslinks, are responsible for SCE formation. Furthermore, all agents but angelicin produced short-lived lesions that led to SCEs in the first period of DNA replication after treatment (twin SCEs), but not in the second (single SCEs). In contrast, angelicin, like methyl methanesulfonate and N-acetoxyacetylaminofluorene, produced lesions that lasted more than one cycle, indicating that several different types of DNA lesions are capable of SCE induction.
为了探究DNA交联是否参与姐妹染色单体交换（SCE）的诱导过程，将中国仓鼠卵巢细胞暴露于两种双功能烷化剂丝裂霉素C和8-甲氧基补骨脂素及其单功能衍生物脱氨甲酰丝裂霉素C和当归素中。数据表明，是单加合物而非交联导致了SCE的形成。此外，除当归素外的所有试剂都会产生短暂的损伤，这些损伤在处理后的DNA复制第一阶段（双SCE）导致SCE形成，但在第二阶段（单SCE）则不会。相比之下，当归素与甲磺酸甲酯和N-乙酰氧基乙酰氨基芴一样，产生的损伤持续超过一个周期，这表明几种不同类型的DNA损伤都能够诱导SCE。
