乙醇对大鼠胃消化活性的影响。

Effect of ethanol on peptic activity in the rat stomach.

作者信息

Puurunen J

出版信息

Digestion. 1982;23(2):97-103. doi: 10.1159/000198694.

DOI:10.1159/000198694

Abstract

The action of ethanol on peptic activity in the stomach was evaluated by studying ethanol-induced changes in pepsinogen secretion in the rat in vivo and the effects of ethanol on pepsinogen and pepsin in vitro. Irrigation of the stomach with 3% ethanol in 100 mM HCl plus 50 mM NaCl had no effect on pepsinogen secretion, whereas 10 and 20% ethanol gave maximal increases of about 40 and 65%, respectively. 10% ethanol in 150 mM NaCl (pH 5.0) stimulated pepsinogen secretion maximally by about 60%. 10% ethanol in distilled water had no effect on spontaneous secretion of pepsinogen, but enhanced pepsinogen secretion induced by irrigation of the stomach with 100 mM HCl, 100 mM NaCl, 150 mM sucrose, or by an intravenous injection of carbachol. Ethanol inhibited the activation of pepsinogen to pepsin in vitro in a pH-dependent manner. The activity of pepsin was inhibited by ethanol with an IC50 value of about 10%. The results indicate that the effects of ethanol on pepsinogen secretion and the enzymes, pepsinogen and pepsin, are opposite in the rat. It is thus difficult to conclude what are the net effects of various concentrations of ethanol on the peptic activity of the gastric content, but at least higher concentrations can be expected to reduce this activity.

摘要

通过研究乙醇对大鼠体内胃蛋白酶原分泌的影响以及乙醇在体外对胃蛋白酶原和胃蛋白酶的作用，评估了乙醇对胃中消化活性的影响。用含100 mM盐酸和50 mM氯化钠的3%乙醇灌胃对胃蛋白酶原分泌没有影响，而10%和20%乙醇分别使胃蛋白酶原分泌量最大增加约40%和65%。含150 mM氯化钠（pH 5.0）的10%乙醇最大程度地刺激胃蛋白酶原分泌，增加约60%。蒸馏水中的10%乙醇对胃蛋白酶原的自发分泌没有影响，但增强了用100 mM盐酸、100 mM氯化钠、150 mM蔗糖灌胃或静脉注射卡巴胆碱诱导的胃蛋白酶原分泌。乙醇在体外以pH依赖的方式抑制胃蛋白酶原激活为胃蛋白酶。乙醇抑制胃蛋白酶活性，IC50值约为10%。结果表明，乙醇对大鼠胃蛋白酶原分泌以及胃蛋白酶原和胃蛋白酶的作用是相反的。因此，很难确定不同浓度乙醇对胃内容物消化活性的净效应是什么，但至少可以预期较高浓度会降低这种活性。