Hällgren R, Stålenheim G
Immunology. 1978 Jul;35(1):13-20.
The phagocytosis of complexes between staphylococcal protein A and 125I-labelled IgG is enhanced by fresh human serum and to a somewhat lesser extent by heat-inactivated serum. Opsonizing activity is detected in two distinct peaks by Sephadex G-200 column chromatography. One peak corresponds to the excluded material and the other to the ascending limb of the second protein peak. The active material in serum is retained on a protein A-Sepharose but not on an IgG-Sepharose column. Active material eluted from a protein A-Sepharose column and run on a Sephadex G-200 column again shows two activity peaks corresponding to those obtained on chromatography of whole serum. The first peak contains mainly IgA nd IgM but very little total protein material. The second peak contains mainly IgG but also IgA. Purified normal IgA, but not one myeloma IgA preparation tested, has the capacity to enhance phagocytosis of protein A-IgG complexes. Possible examinations for the opsonin activity are discussed.
新鲜人血清可增强葡萄球菌蛋白A与125I标记的IgG之间复合物的吞噬作用，热灭活血清的增强作用稍弱。通过Sephadex G - 200柱色谱法可检测到调理活性的两个不同峰。一个峰对应于被排除的物质，另一个峰对应于第二个蛋白质峰的上升支。血清中的活性物质保留在蛋白A - 琼脂糖柱上，而不保留在IgG - 琼脂糖柱上。从蛋白A - 琼脂糖柱洗脱的活性物质再次在Sephadex G - 200柱上运行时，又显示出与全血清色谱分析所得的两个活性峰相对应。第一个峰主要含有IgA和IgM，但总蛋白物质很少。第二个峰主要含有IgG，但也含有IgA。纯化的正常IgA（但所测试的一种骨髓瘤IgA制剂不具有此能力）具有增强蛋白A - IgG复合物吞噬作用的能力。文中讨论了对调理素活性的可能检测方法。
