Petersson B A, Stålenheim G
Immunology. 1977 May;32(5):623-33.
The ability of human leucocytes to release histamine on protein A treatment is lost when the cells are washed repeatedly. It is, however, possible to restore the sensitivity to protein A treatment by incubating the leucocytes in serum. Treatment of the cells with purified IgG does not restore the activity. The material responsible for the resensitization is eluted both in the second and the third protein peak when serum is chromatographed on Sephadex G-200, indicating the possible existence of several active factors. Material with low immunoglobulin content, but with retained capacity to resensitize leucocytes to release histamine on protein A treatment, was obtained by repeated chromatography of peak III material on the Sephadex G-200 column. Furthermore, material from the second and third peaks from Sephadex G-200 deprived of their IgG by passage through a protein A Sepharose or a DEAE-cellulose column had the same capacity to resensitize the leucocytes as unseparated material. When serum was separated by Pevikon block electrophoresis, most of the activity was detected in the alpha and beta regions but only little in the gamma region. The serum fractionations indicate that neither IgG nor the other immunoglobulins are the factor(s) responsible for resensitizing the leucocytes to release histamine on protein A treatment. Beside being necessary for protein A-induced histamine release, the factor (or factors) is also essential for protein A-induced desensitization of human leucocytes.
当人类白细胞被反复洗涤后，其在蛋白A处理下释放组胺的能力会丧失。然而，通过将白细胞在血清中孵育，可以恢复对蛋白A处理的敏感性。用纯化的IgG处理细胞并不能恢复活性。当血清在葡聚糖凝胶G - 200上进行层析时，负责重新致敏的物质在第二个和第三个蛋白峰中都被洗脱出来，这表明可能存在几种活性因子。通过将峰III物质在葡聚糖凝胶G - 200柱上反复层析，获得了免疫球蛋白含量低但仍保留使白细胞在蛋白A处理下释放组胺的重新致敏能力的物质。此外，来自葡聚糖凝胶G - 200的第二个和第三个峰的物质，通过蛋白A琼脂糖或二乙氨基乙基纤维素柱去除其IgG后，与未分离的物质具有相同的使白细胞重新致敏的能力。当血清通过聚乙烯醇缩丁醛区带电泳进行分离时，大部分活性在α和β区域被检测到，而在γ区域只有很少活性。血清分级分离表明，无论是IgG还是其他免疫球蛋白都不是使白细胞在蛋白A处理下释放组胺的重新致敏因子。除了对蛋白A诱导的组胺释放是必需的外，该因子（或这些因子）对蛋白A诱导的人类白细胞脱敏也是必不可少的。
