Lopez L A, Bertini F
J Exp Zool. 1982 May 20;221(1):9-12. doi: 10.1002/jez.1402210103.
The tubulin of both brain and testis of the toad Bufo arenarum was partially depolymerized when the animals were maintained on ice for 90 minutes or 17 hours. Recovery of the animals at room temperature restored the normal degree of polymerization in both organs. Normalization of the polymerization degree also occurred in organs separated from cooled animals and incubated in vitro for 90 minutes at 20 degrees C. When the organs were cooled in vitro, only testicular tubulin underwent depolymerization, and in a degree similar to that occurring by incubation at 20 degrees C. The results suggest that the mechanisms regulating tubulin depolymerization in vivo are different from those taking place in vitro.
当蟾蜍（Bufo arenarum）在冰上放置90分钟或17小时时，其脑和睾丸中的微管蛋白会部分解聚。在室温下恢复后，两个器官中的聚合度恢复正常。从冷却的动物身上分离出的器官在20℃体外孵育90分钟后，聚合度也恢复正常。当器官在体外冷却时，只有睾丸微管蛋白发生解聚，且解聚程度与在20℃孵育时相似。结果表明，体内调节微管蛋白解聚的机制与体外不同。
