McVeigh J M, Tarrant P V
J Nutr. 1982 Jul;112(7):1306-14. doi: 10.1093/jn/112.7.1306.
The effect of type of diet and fasting on resting muscle glycogen concentration and on rate of recovery after glycogen depletion was studied in beef M. logissimus dorsi. Hereford heifers were fed either barley or hay, or were fasted for 9 days. Resting muscle glycogen concentration was significantly increased by feeding barley and decreased by fasting, relative to the value in cattle on a maintenance diet of hay. Rates of repletion of muscle glycogen following depletion by adrenalin injection averaged 7.6 mumol/g per day on barley, 6.1 mumol/g per day on hay and 1.5 mumol/g per day in fasted heifers. On refeeding the fasted group with hay the rate of glycogen reported for nonruminant species. The specific activity of muscle glycogen synthase (I + D) was 2.13 mumol/minute per gram of wet tissue, and normal conversion to the active (I) form was observed in glycogen-depleted beef muscles. Determination of blood glucose, plasma insulin and muscle glucose 6-phosphate concentrations provided some evidence for a decreased availability of glycogen precursors during the period of muscle glycogen resynthesis. It was concluded that a lower glucose availability in cattle compared with nonruminant species caused a slower rate of muscle glycogen repletion. Different repletion rates between the experimental groups reflected different levels of energy intake and corresponding changes in glucose utilization rates.
在牛肉背最长肌中研究了日粮类型和禁食对静息肌肉糖原浓度以及糖原耗竭后恢复速率的影响。给赫里福德小母牛饲喂大麦或干草，或禁食9天。与维持日粮为干草的牛相比，饲喂大麦可显著提高静息肌肉糖原浓度，禁食则使其降低。通过注射肾上腺素耗竭糖原后，肌肉糖原补充速率在饲喂大麦的小母牛中平均为每天7.6 μmol/g，在饲喂干草的小母牛中为每天6.1 μmol/g，在禁食的小母牛中为每天1.5 μmol/g。给禁食组重新饲喂干草后，糖原补充速率与非反刍动物报道的速率相似。肌肉糖原合酶（I + D）的比活性为每克湿组织2.13 μmol/分钟，并且在糖原耗竭的牛肉肌肉中观察到正常转化为活性（I）形式。测定血糖、血浆胰岛素和肌肉葡萄糖6-磷酸浓度为肌肉糖原再合成期间糖原前体可用性降低提供了一些证据。得出的结论是，与非反刍动物相比，牛体内葡萄糖可用性较低导致肌肉糖原补充速率较慢。实验组之间不同的补充速率反映了能量摄入水平的差异以及葡萄糖利用率的相应变化。
