Analysis of cellular reaction to glucose of toad skin treated with aldosterone.

Toad skin exposed overnight to aldosterone, was examined by means of microelectrodes before and after addition of glucose to the incubation fluid in 7 instances. Glucose addition led with minutes to epithelial cell hyperpolarization associated with increases of conductances at both cell borders, the increase being twice as large (in relative terms) at the basolateral border as at the apical one. Since glucose had no effect on untreated preparations, these observations represent another argument for aldosterone influencing target cells in a way that is not restricted to increased apical conductance.