来自枯草芽孢杆菌168的赖氨酸转运RNA：同功受体在兔网织红细胞无细胞蛋白质合成系统中的功能。
Lysine tRNAs from Bacillus subtilis 168: function of the isoacceptors in a rabbit reticulocyte cell-free protein-synthesizing system.
作者信息
Smith D W, McNamara A L, Vold B S
出版信息
Nucleic Acids Res. 1982 May 25;10(10):3117-23. doi: 10.1093/nar/10.10.3117.
The two principal tRNA Lys isoaccepting species of Bacillus subtilis were compared in their functional activity in translating rabbit globin. Although neither species demonstrates any preference in reading either of the lysine codons, there is an overall preference for tRNa Lys 3 in lysine incorporation. The ratios of lysine incorporated by the two species into the different lysine-containing sites in the globin subunits vary over a more than two-fold range. As described in the accompanying paper, tRNA Lys 1 is a hypomodified form of tRNA Lys 3. Consistent with studies on other rRNA species, the fully modified isoacceptor functions preferentially. In contrast to these results, however, the fully modified isoacceptor (tRNA Lys 3) is found predominantly in rapidly dividing cells while the hypomodified isoacceptor (tRNA Lys 1) predominates in the stationary cells and spores of B.l subtilis.
对枯草芽孢杆菌的两种主要异源接受性赖氨酸tRNA在翻译兔珠蛋白中的功能活性进行了比较。虽然这两种tRNA在读取任何一种赖氨酸密码子时都没有表现出偏好，但在赖氨酸掺入过程中总体上更倾向于tRNA Lys 3。这两种tRNA将赖氨酸掺入珠蛋白亚基中不同含赖氨酸位点的比例变化范围超过两倍。如随附论文所述，tRNA Lys 1是tRNA Lys 3的低修饰形式。与对其他tRNA种类的研究一致，完全修饰的异源接受体优先发挥功能。然而，与这些结果相反，完全修饰的异源接受体（tRNA Lys 3）主要存在于快速分裂的细胞中，而低修饰的异源接受体（tRNA Lys 1）在枯草芽孢杆菌的静止细胞和孢子中占主导地位。
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