Olsen C E, Penhoet E E
Biochemistry. 1976 Oct 19;15(21):4649-54. doi: 10.1021/bi00666a016.
Chromatographic and functional properties of tyrosine isoaccepting transfer ribonucleic acids (tRNAs) from placenta and HeLa cells were analyzed and compared. RPC-5 chromatography separated four major isoacceptors from each source, with those from HeLa cells eluting generally later than those from placenta. There was some overlap: HeLa tRNA1Tyr eluted in a position between placenta tRNA3Tyr and tRNA4Tyr; and HeLa tRNA2Tyr and placenta tRNA4Tyr eluted in similar positions with the HeLa isoacceptor eluting slightly later than the placental isoacceptor. Thus there are no isoacceptors common to both sources. The function of the individual isoacceptors was compared in a rabbit-reticulocyte, cell-free, protein-synthesizing system for both the rate of incorporation of tyrosine into the polypeptide chain and the site of incorporation in alpha-globin. Two isoacceptors were compared simultaneously in the same reaction, and overlapping comparisons were made to relate each isoacceptor to all the others. There were no significant differences in the rates of incorporation among the isoacceptors, nor were there any differences in the sites of incorporation. All eight isoacceptors donated tyrosine equally well into the three tyrosine containing tryptic peptides of alpha-globin. Whatever the structural differences among the tyrosine isoacceptors are, they do not affect the function of the tRNA in this protein-synthesizing system.
对来自胎盘和海拉细胞的酪氨酸同工受体转移核糖核酸（tRNA）的色谱特性和功能特性进行了分析和比较。RPC - 5色谱法从每个来源分离出四种主要的同工受体，海拉细胞来源的同工受体洗脱时间通常比胎盘来源的晚。存在一些重叠：海拉细胞的tRNA1Tyr在胎盘的tRNA3Tyr和tRNA4Tyr之间的位置洗脱；海拉细胞的tRNA2Tyr和胎盘的tRNA4Tyr在相似位置洗脱，海拉细胞的同工受体洗脱时间略晚于胎盘的同工受体。因此，这两个来源没有共同的同工受体。在兔网织红细胞无细胞蛋白质合成系统中，比较了各个同工受体在酪氨酸掺入多肽链的速率以及在α - 珠蛋白中的掺入位点方面的功能。在同一反应中同时比较了两种同工受体，并进行了重叠比较以将每个同工受体与其他所有同工受体联系起来。同工受体之间在掺入速率上没有显著差异，在掺入位点上也没有差异。所有八种同工受体将酪氨酸同样良好地掺入到α - 珠蛋白的三个含酪氨酸的胰蛋白酶肽段中。无论酪氨酸同工受体之间的结构差异如何，它们在这个蛋白质合成系统中都不影响tRNA的功能。
