蛋白酶催化的肽酰肼合成。
The proteinase-catalysed synthesis of peptide hydrazides.
作者信息
Jones R M, Offord R E
出版信息
Biochem J. 1982 Apr 1;203(1):125-9. doi: 10.1042/bj2030125.
Abstract
- We report the trypsin-catalysed conversion, in high yield, of peptides to peptide hydrazides, t-butyloxycarbonylhydrazides and phenylhydrazides. The substitution is at the alpha-carboxy group. 2. We discuss the relative merits of carrying out the conversion either simultaneously with tryptic cleavage of the parent protein or after such cleavage. 3. We report analogous results with chymotrypsin, elastase and subtilisin. 4. We propose the use of such products in protein semi-synthesis and in the preparation of specific proteinase inhibitors.
摘要
- 我们报道了胰蛋白酶催化肽高效转化为肽酰肼、叔丁氧羰基酰肼和苯酰肼的过程。取代发生在α-羧基上。2. 我们讨论了在对亲本蛋白质进行胰蛋白酶切割的同时进行转化或在切割后进行转化的相对优点。3. 我们报道了胰凝乳蛋白酶、弹性蛋白酶和枯草杆菌蛋白酶的类似结果。4. 我们建议将此类产物用于蛋白质半合成及特定蛋白酶抑制剂的制备。
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本文引用的文献
1
Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.
Nature. 1966 Aug 6;211(5049):591-3. doi: 10.1038/211591a0.
2
The subunit structure of prealbumin.
Biochem J. 1971 Nov;125(1):309-17. doi: 10.1042/bj1250309.
3
The semisynthesis of portions of hen's-egg lysozyme by fragment condensation.
Biochem J. 1976 Dec 1;159(3):487-93. doi: 10.1042/bj1590487.
4
The preparation of protected fragments of lysozyme for semisynthesis.
Biochem J. 1976 Dec 1;159(3):467-79. doi: 10.1042/bj1590467.
5
A second activation peptide from bovine cationic trypsinogen.
Biochem J. 1977 Oct 1;167(1):285-7. doi: 10.1042/bj1670285.
6
Enzymatic resynthesis of the hydrolyzed peptide bond(s) in ribonuclease S.
Biochemistry. 1979 Feb 20;18(4):586-92. doi: 10.1021/bi00571a006.
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