Heddle R J, La Brooy J T, Shearman D J
Clin Exp Immunol. 1982 May;48(2):469-76.
Mononuclear cells were isolated from the mucosa and submucosa of small intestine and colon of 22 subjects with localized, anatomically remote disease and four subjects with Crohn's disease (nine specimens) by sequential treatment with EDTA and collagenase. The effects of isolation techniques on cell yields and viability were examined. Secretion of specific IgA, IgM and IgG antibodies to common faecal Escherichia coli strains by individual mononuclear cells was studied using a haemolytic plaque assay. A majority of specific antibody secreting cells secreted IgA antibody. This response was greatest and most consistent in the distal colon but extended from stomach to rectum. There was no evidence of a primary defect in IgA antibody response in the few subjects with Crohn's disease available for study.
通过用乙二胺四乙酸（EDTA）和胶原酶依次处理，从22名患有局限性、解剖学上远处疾病的受试者以及4名患有克罗恩病的受试者（9个标本）的小肠和结肠黏膜及黏膜下层分离出单核细胞。研究了分离技术对细胞产量和活力的影响。使用溶血空斑试验研究了单个单核细胞对常见粪便大肠杆菌菌株的特异性IgA、IgM和IgG抗体的分泌情况。大多数分泌特异性抗体的细胞分泌IgA抗体。这种反应在远端结肠中最为强烈且最为一致，但从胃延伸至直肠。在少数可供研究的克罗恩病受试者中，没有证据表明IgA抗体反应存在原发性缺陷。
