Bekoff M C, Levine H, Schlossman S F
J Immunol. 1982 Sep;129(3):1173-80.
The immune response to the chemically defined DNP-oligo-L-lysine antigens is under Ir gene control in the guinea pig. Strain 2 (responder) guinea pigs mount a highly specific T cell-mediated and humoral immune response capable of discriminating closely related DNP-peptides. In contrast, strain 13 (nonresponder) animals fail to mount a cell-mediated response, and produce antibody that is only DNP-specific. In these studies a common idiotypic determinant is defined on highly specific anti-epsilon,DNP-Lys10 antibody produced by strain 2 guinea pigs. Ir gene nonresponder antibody is idiotype-negative. The presence of the idiotypic determinant distinguishes responder anti-epsilon,DNP-Lys10 antibody from responder antibodies elicited by structurally related antigens such as epsilon,DNP-Lys9 and alpha,DNP-Lys10-. Investigation of the regulation of idiotype expression demonstrated that production of idiotype-positive antibody requires the presence of viable, antigen-responsive T cells. Moreover, genetic analysis revealed that expression of the shared idiotype correlates directly with the phenotypic expression of I region genes (DTH responsiveness, Ia antigens) in (2 x 13)F1 x 13 backcross and randomly bred Hartley guinea pigs. Thus, Ir gene regulation of the immune response may be reflected in the v region specificities expressed by antigen-specific B cell clones.
豚鼠对化学合成的二硝基苯基 - 寡聚 -L-赖氨酸抗原的免疫反应受Ir基因控制。2系（反应者）豚鼠能引发高度特异性的T细胞介导和体液免疫反应，能够区分密切相关的二硝基苯基肽。相比之下，13系（无反应者）动物无法引发细胞介导的反应，仅产生二硝基苯基特异性抗体。在这些研究中，在2系豚鼠产生的高度特异性抗ε，二硝基苯基 - 赖氨酸10抗体上定义了一个共同的独特型决定簇。Ir基因无反应者抗体为独特型阴性。独特型决定簇的存在将反应者抗ε，二硝基苯基 - 赖氨酸10抗体与由结构相关抗原如ε，二硝基苯基 - 赖氨酸9和α，二硝基苯基 - 赖氨酸10 - 引发的反应者抗体区分开来。对独特型表达调控的研究表明，独特型阳性抗体的产生需要有存活的、对抗原产生反应的T细胞。此外，遗传分析显示，在（2×13）F1×13回交和随机繁殖的哈特利豚鼠中，共享独特型的表达与I区基因的表型表达（迟发型超敏反应性、Ia抗原）直接相关。因此，免疫反应的Ir基因调控可能反映在抗原特异性B细胞克隆所表达的V区特异性上。
