Campos-Neto A, Schlossman S F, Levine H, Yanovski A, Yaron A
Immunology. 1978 Nov;35(5):763-9.
A series of 2,4-dinitrophenyl (DNP) octapeptides containing L-lysine and L-alanine were prepared to examine the specificity and genetic restrictions of both cellular and humoral immune responses in inbred guinea-pigs. Strains 2 and 13 guinea-pigs were therefore immunized with Lys4-Ala3-Lys(DNP), Lys3Ala4-Lys(DNP), Lys2-Ala5-Lys(DNP) and Lys-Ala6-Lys(DNP). Only Lys4-Ala3-Lys(DNP) was under Ir gene control and could induce both antibody and T-cell responses in strain 2 guinea-pigs. In contrast, Lys4-Ala3-Lys(DNP) injected in strain 13 guinea-pigs and the other DNP-octapeptides injected in strain 2 or 13 guinea-pigs elicited only antibody formation and no specific T-cell mediated response. Antibody formed in the absence of specific T-cell responsiveness in either strain 2 or 13 was hapten specific and lacked the capacity to discriminate the immunizing antigen from closely related DNP-peptides. Antibody produced by animals with specific T-cell responses, on the other hand, was exquisitely specific for the immunizing peptide and could discriminate it from closely related peptides.
制备了一系列含有L-赖氨酸和L-丙氨酸的2,4-二硝基苯基（DNP）八肽，以研究近交系豚鼠细胞免疫和体液免疫反应的特异性及遗传限制。因此，用Lys4-Ala3-Lys(DNP)、Lys3Ala4-Lys(DNP)、Lys2-Ala5-Lys(DNP)和Lys-Ala6-Lys(DNP)对2系和13系豚鼠进行免疫。只有Lys4-Ala3-Lys(DNP)受Ir基因控制，能在2系豚鼠中诱导抗体和T细胞反应。相比之下，给13系豚鼠注射Lys4-Ala3-Lys(DNP)以及给2系或13系豚鼠注射其他DNP-八肽仅诱导抗体形成，未引发特异性T细胞介导的反应。在2系或13系豚鼠中，在无特异性T细胞反应性的情况下形成的抗体是半抗原特异性的，缺乏区分免疫抗原与密切相关DNP-肽的能力。另一方面，具有特异性T细胞反应的动物产生的抗体对免疫肽具有高度特异性，能够将其与密切相关的肽区分开来。
