Nakashio S, Nakanishi T, Koshikawa T, Nishihara T, Ichikawa T, Kondo M
Microbios. 1982;33(132):73-80.
An unidentified ninhydrin-positive substance was detected in trichloroacetic acid extract from the sporulating cells of Bacillus subtilis NRRL B558. The substance was isolated as the corresponding 2,4-dinitrophenyl (DNP) derivative, the structure of which was identified with a pyridinium salt of DNP-taurine, based on spectral and synthetic evidence. a pyridine molecule should be introduced into a taurine molecule during the isolation procedure and hence, the original amino acid was assigned to taurine. Taurine was accumulated into the cells from the medium during the early stages os sporulation, and thereafter metabolized or excreted.
在枯草芽孢杆菌NRRL B558芽孢形成细胞的三氯乙酸提取物中检测到一种未鉴定的茚三酮阳性物质。该物质被分离为相应的2,4-二硝基苯基（DNP）衍生物，根据光谱和合成证据，其结构被鉴定为DNP-牛磺酸的吡啶盐。在分离过程中，一个吡啶分子应引入到牛磺酸分子中，因此，原始氨基酸被确定为牛磺酸。在芽孢形成的早期阶段，牛磺酸从培养基中积累到细胞中，然后被代谢或排泄。
