Chow C T, Takahashi I
J Bacteriol. 1972 Mar;109(3):1175-80. doi: 10.1128/jb.109.3.1175-1180.1972.
An asporogenous mutant of Bacillus subtilis Sp(-)H12-3, which is considered to have a block at stage 0, showed general growth characteristics similar to those of sporulating cultures. However, a sudden increase in the total amount of acid-soluble nucleotides observed at t(2) in sporulating bacteria was completely absent in this mutant. In sporulating cells, a marked increase in two nucleotides which were identified to be uridine diphosphate (UDP)-galactose and UDP-N-acetylglucosamine was noted, whereas UDP-glucose appeared to be accumulated in the mutant cells at t(2). No unusual nucleotides were found in the strains of B. subtilis examined. The possible role of these UDP derivatives in early stages of sporulation in B. subtilis is discussed.
枯草芽孢杆菌Sp(-)H12-3的一个不产芽孢突变体被认为在第0阶段存在阻断，其表现出与产芽孢培养物相似的一般生长特征。然而，在产芽孢细菌中于t(2)观察到的酸溶性核苷酸总量的突然增加在该突变体中完全不存在。在产芽孢细胞中，注意到两种被鉴定为尿苷二磷酸（UDP）-半乳糖和UDP-N-乙酰葡糖胺的核苷酸显著增加，而UDP-葡萄糖似乎在t(2)时在突变体细胞中积累。在所检测的枯草芽孢杆菌菌株中未发现异常核苷酸。讨论了这些UDP衍生物在枯草芽孢杆菌芽孢形成早期阶段可能的作用。
