Imagawa M, Ichikawa T, Kondo M
Microbiol Immunol. 1982;26(3):181-90. doi: 10.1111/j.1348-0421.1982.tb00170.x.
Hg- and Cd-spores of Bacillus megaterium QM B1551 were produced in Schaeffer's medium containing mercuric chloride and cadmium chloride respectively. Metals were added to the medium at 9 hr of incubation (Stage V) to give a final concentration of 50 microM. It was found by electron microscopic and biochemical studies that the coats of both Hg- and Cd-spores were thinner than those of control spores. Of the total Hg and Cd in the spores, 77% of the Hg and 63% of the Cd were detected in the spore coats. Hg- and Cd-spores were less resistant to heat and more sensitive to germinants than control spores. Other properties of Hg- and Cd-spores were similar to those of control spores. These results suggest that the spore coat has some relationship to the heat resistance and germinability of spores.
巨大芽孢杆菌QM B1551的汞孢子和镉孢子分别在含有氯化汞和氯化镉的谢弗培养基中产生。在培养9小时（第五阶段）时将金属添加到培养基中，使其终浓度达到50微摩尔。通过电子显微镜和生化研究发现，汞孢子和镉孢子的孢壁均比对照孢子的孢壁薄。在孢子中的总汞和镉中，77%的汞和63%的镉在孢壁中被检测到。汞孢子和镉孢子比对照孢子耐热性更低，对萌发剂更敏感。汞孢子和镉孢子的其他特性与对照孢子相似。这些结果表明，孢壁与孢子的耐热性和可萌发能力存在某种关系。
