巨大芽孢杆菌孢子的萌发与肽聚糖溶解
Germination and peptidoglycan solubilization in Bacillus megaterium spores.
作者信息
Hsieh L K, Vary J C
出版信息
J Bacteriol. 1975 Aug;123(2):463-70. doi: 10.1128/jb.123.2.463-470.1975.
Abstract
During initiation of Bacillus megaterium QM B1551 spore germination, trichloroacetic acid-soluble, nondialyzable peptidoglycan fragments with an average molecular weight of 20,000 were excreted. This solubilization of peptidoglycan was measured in vitro as the amount of trichloroacetic acid-soluble hexosamine released from a suspension of broken spores. HgC12, a potent inhibitor of initiation, had no effect on the in vitro solubilization of peptidoglycan. In vivo, HgC12 had no effect on peptidoglycan release from spores that had lost heat resistance, but HgC12 did block complete absorbance loss. These results suggest that mercury inhibits some reactions that normally occur before loss in heat resistance but not the subsequent peptidoglycan release, and mercury inhibits other reactions involved with complete absorbance loss.
摘要
在巨大芽孢杆菌QM B1551孢子萌发起始阶段，会分泌出平均分子量为20,000的三氯乙酸可溶性、不可透析的肽聚糖片段。肽聚糖的这种溶解在体外通过从破碎孢子悬液中释放的三氯乙酸可溶性己糖胺的量来测定。起始的强效抑制剂HgC12对肽聚糖的体外溶解没有影响。在体内，HgC12对已失去耐热性的孢子释放肽聚糖没有影响，但HgC12确实会阻止吸光度完全丧失。这些结果表明，汞抑制了一些通常在耐热性丧失之前发生的反应，但不抑制随后的肽聚糖释放，并且汞抑制了与吸光度完全丧失相关的其他反应。
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