Liber H L, Thilly W G
Mutat Res. 1982 Jun;94(2):467-85. doi: 10.1016/0027-5107(82)90308-6.
A thymidine kinase heterozygote was isolated from a diploid human lymphoblast line which forms colonies with high efficiency in microtiter dishes. We show that this cell line, called TK6, can be mutated from a TK+/- to TK-/- state by diverse mutagens, including ethyl methanesulfonate, butyl methanesulfonate, nitrosomethylurea, UV light, ICR-191, 4-nitroquinoline oxide, fluorodeoxyuridine, benzo[a]pyrene and aflatoxin B1. We report here the experiments required to demonstrate the applicability of this new line in quantitative assays of mutation in human cells. Mitotic recombination between the centromere and the tk locus could not be induced by either dimethylsulfoxide or phorbol-12-myristate-13-acetate.
从一个在微量滴定板中能高效形成集落的二倍体人类淋巴母细胞系中分离出了一个胸苷激酶杂合子。我们发现，这个名为TK6的细胞系可以被多种诱变剂从TK+/-状态突变为TK-/-状态，这些诱变剂包括甲磺酸乙酯、甲磺酸丁酯、亚硝基甲基脲、紫外线、ICR-191、4-硝基喹啉氧化物、氟脱氧尿苷、苯并[a]芘和黄曲霉毒素B1。我们在此报告了为证明该新细胞系在人类细胞突变定量测定中的适用性而进行的实验。二甲亚砜或佛波醇-12-肉豆蔻酸酯-13-乙酸酯均不能诱导着丝粒与tk基因座之间的有丝分裂重组。
