牛接种布鲁氏菌流产亚种疫苗并受到攻击后的血清学反应。

Serological response of cattle after vaccination and challenge with Brucella abortus.

作者信息

Sutherland S S, Le Cras D V, Robertson A G, Johnston J M, Evans R J

出版信息

Vet Microbiol. 1982 May;7(2):165-75. doi: 10.1016/0378-1135(82)90028-1.

Abstract

New and currently used serological procedures were evaluated using sera from cattle that were challenged with B. abortus S544 (S544) after vaccination with either B. abortus S19 (S19) or B. abortus 45/20 (S45/20) as calves or adults. In animals vaccinated with S19, titres to the indirect haemolysis test (IHLT) rose more slowly, declined more rapidly and involved fewer animals than did titres to the complement fixation test (CFT). In animals vaccinated with S45/20 the rough antigen complement fixation test (RCFT) showed persistent titres. At slaughter the IHLT and CFT were found to be more specific and more sensitive than the Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) in the detection of cattle infected with B. abortus.

摘要

使用牛血清对新的和目前使用的血清学检测方法进行了评估，这些牛在犊牛期或成年期接种了流产布鲁氏菌S19（S19）或流产布鲁氏菌45/20（S45/20）后，又用流产布鲁氏菌S544（S544）进行了攻毒。在接种S19的动物中，间接溶血试验（IHLT）的滴度上升更缓慢，下降更迅速，涉及的动物数量比补体结合试验（CFT）的滴度少。在接种S45/20的动物中，粗糙抗原补体结合试验（RCFT）显示滴度持续存在。在屠宰时发现，在检测感染流产布鲁氏菌的牛时，IHLT和CFT比玫瑰红平板试验（RBPT）和血清凝集试验（SAT）更特异、更敏感。