Sutherland S S
Vet Microbiol. 1983 Aug;8(4):405-9. doi: 10.1016/0378-1135(83)90053-6.
Fifty-four cattle were sensitised to Brucella antigens either by vaccination with Brucella abortus strain 19 (S19) or B. abortus 45/20 (S45/20) and 24 of these were challenged 12 weeks after mating with virulent B. abortus strain 544 (S544). A further 12 cattle which were not vaccinated were exposed to S544. After 40 weeks, all these cattle (66), together with 5 cattle which were not sensitised by vaccination or challenge were subsequently inoculated with one dose of S45/20 and the anamnestic response was measured by the complement fixation test. Ten to 15 weeks later the cattle were slaughtered and tissues cultured. Of the 52 (2 died) vaccinated cattle, 35 gave a positive anamnestic response and 20 of these were not challenged. Of the 17 unvaccinated cattle, one gave a positive response and this animal had been exposed to S544 prior to the inoculation with S45/20. The results indicated that the method had a level of sensitivity of 75% and specificity of 100% in serologically negative cattle that had been exposed previously to Brucella antigens. An evaluation of the method for detecting serologically negative, but infected cattle was not possible as the number of cattle suitable for examination in this study was too low.
54头奶牛通过接种流产布鲁氏菌19号菌株（S19）或流产布鲁氏菌45/20菌株（S45/20）对布鲁氏菌抗原产生致敏，其中24头在配种12周后用强毒流产布鲁氏菌544菌株（S544）进行攻毒。另外12头未接种疫苗的奶牛暴露于S544。40周后，所有这些奶牛（66头），连同5头未通过接种疫苗或攻毒致敏的奶牛，随后接种一剂S45/20，并通过补体结合试验测定回忆反应。10至15周后，屠宰这些奶牛并对组织进行培养。在52头（2头死亡）接种疫苗的奶牛中，35头产生了阳性回忆反应且其中20头未受到攻毒。在17头未接种疫苗的奶牛中，1头产生了阳性反应，这头牛在接种S45/20之前已暴露于S544。结果表明，该方法在先前已暴露于布鲁氏菌抗原的血清学阴性奶牛中，敏感性水平为75%，特异性为100%。由于本研究中适合检查的奶牛数量过低，无法对检测血清学阴性但已感染奶牛的方法进行评估。
