Chappel R J, Hayes J, Rogerson B A, Shenfield L J
J Hyg (Lond). 1982 Feb;88(1):11-9. doi: 10.1017/s0022172400069849.
Serum sample were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests. The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions. In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera. Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.
从281头接种布鲁氏菌19号菌株的小母牛以及50头接受过两次45/20号布鲁氏菌死菌佐剂（K45/20A）疫苗注射的小母牛身上采集血清样本。通过布鲁氏菌病放射免疫测定法（RIA）测得的血清学反应与通过其他检测方法测得的反应进行比较。发现19号菌株疫苗接种后头几周内牛的血清学反应对持续性反应的频率几乎没有指导意义。对于19号菌株，持续性反应被认为是在接种疫苗12个月或更长时间后出现的反应。在按推荐年龄接种疫苗的小母牛中，RIA检测出少量持续性反应（374份血清中有4份），热固定补体结合试验（CFTW）（383份中有6份）、冷固定补体结合试验（185份中有1份）、血清凝集试验（222份中有2份）和间接溶血试验（IHLT）（369份中有2份）。虎红平板试验在374份血清中检测出74例持续性反应。50头小母牛中有5头对K45/20A疫苗的反应特别持久。在这些动物中，RIA和IHLT保持阳性的时间比CFTW更长。
