Hifny A, Hassan A S, Kamel G, Abo-Elmagd A
Z Mikrosk Anat Forsch. 1982;96(2):303-17.
A histomorphological examination has been made in the pars distalis of the pituitaries of twelve normal healthy sexually mature ewes which were classified into three groups (resting, follicular and luteal phase) according to the different phases of reproduction. The histological features of FSH- and LH-cells during anestrus has been used to establish a standard with which the subsequent changes during the follicular and luteal phases were compared. Using PAS/OG, AB/OG and PFA/AB/PAS/OG stains, the FSH- and LH-cells were differentiated and identified from the other forms of the pars distalis cells. The topographical localization of the FSH- and LH-cells, in various regions and levels in the pars distalis of balady ewe as well as their frequencies during the different phases of reproductive cycle were discussed. Statistical analysis of the different cell count during the different phases of estrus cycle in comparison with the resting phase (anestrus), can be summarized as follows: - The FSH- and LH-cells are more pronounced, more active and significantly increased in number during the follicular phase of estrus cycle. - The LH-cells appear to be more abundant and show sings of hyperactivity at the luteal phase than the resting and follicular phases.
对12只性成熟的正常健康母羊的垂体远侧部进行了组织形态学检查，根据生殖的不同阶段将其分为三组（静止期、卵泡期和黄体期）。利用发情间期促卵泡激素（FSH）和促黄体生成素（LH）细胞的组织学特征建立了一个标准，以此来比较卵泡期和黄体期随后发生的变化。使用过碘酸雪夫氏染色法/橙黄G染色法（PAS/OG）、爱尔新蓝染色法/橙黄G染色法（AB/OG）和多聚甲醛固定法/爱尔新蓝染色法/过碘酸雪夫氏染色法/橙黄G染色法（PFA/AB/PAS/OG），从垂体远侧部的其他细胞类型中区分并鉴定出FSH和LH细胞。讨论了本地母羊垂体远侧部不同区域和水平的FSH和LH细胞的地形定位，以及它们在生殖周期不同阶段的频率。发情周期不同阶段与静止期（发情间期）相比，不同细胞计数的统计分析结果总结如下：- 在发情周期的卵泡期，FSH和LH细胞更为明显、更活跃，数量显著增加。- 与静止期和卵泡期相比，LH细胞在黄体期似乎更为丰富，且表现出活跃过度的迹象。
