[肠球菌DNA的核苷酸组成]

[Nucleotide composition of enterococcal DNA].

作者信息

Sedov V I, Levanova G F

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1982 Jun(6):38-40.

Abstract

A simple method for isolating DNA from enterococci has been developed. This method allows one to extract DNA of good quality in optimal amounts irrespective of the method used for obtaining the culture and the duration of its incubation. Mobile enterococci are sharply differentiated into 3 groups in accordance with their nucleotide composition, the content of GC pairs in DNA being 31.6% in group 1, 35.8-38.0% in group 2, and 40.6-41.5% in group 3.

摘要

已开发出一种从肠球菌中分离DNA的简单方法。该方法能够使人们提取出质量优良且数量合适的DNA，而不论用于获取培养物的方法及其培养持续时间如何。游动肠球菌根据其核苷酸组成可明显分为3组，第1组DNA中GC对的含量为31.6%，第2组为35.8 - 38.0%，第3组为40.6 - 41.5%。