Agostino M, Prowse S J, Lafferty K J
Aust J Exp Biol Med Sci. 1982 Apr;60(Pt 2):219-22. doi: 10.1038/icb.1982.26.
Using preliminary in vitro culture in an oxygen-rich atmosphere to facilitate survival of the graft, long term survival (greater than 100 days) of mouse pancreatic islet allografts was obtained subsequently in non-immunosuppressed diabetic animals. When anti-donor alloantiserum was transferred to these animals, the grafts were not rejected despite the fact that circulating cytotoxic antibody was demonstrated up to 7 days after injection of alloantiserum. Similarly, there was no graft rejection following the injection of anti-donor alloantiserum and complement. These results show that cultured islet allografts are not susceptible to antibody and complement.
通过在富氧环境中进行初步体外培养以促进移植物存活，随后在未进行免疫抑制的糖尿病动物中实现了小鼠胰岛同种异体移植物的长期存活（超过100天）。当将抗供体同种异体抗血清转移至这些动物时，尽管在注射同种异体抗血清后长达7天检测到循环细胞毒性抗体，但移植物并未被排斥。同样，在注射抗供体同种异体抗血清和补体后也没有发生移植物排斥。这些结果表明，培养的胰岛同种异体移植物不易受到抗体和补体的影响。
