Kootstra A, MacLeod M C, Iyer R, Selkirk J K, Slaga T J
Carcinogenesis. 1982;3(7):821-4. doi: 10.1093/carcin/3.7.821.
Metabolites of benzo[a]pyrene (B[a]P) have been shown to modify chromosomal proteins with great specificity. Using the (+) and (-) enantiomers of anti-B[a]P diol epoxide to label isolated nuclei we found a remarkable difference in the capacity of these two compounds to modify histones H2A and H3. The (+) enantiomer modified histones H2A and H3, while the (-) enantiomer, which was shown to modify mainly histone H2A, had a much lower affinity for histone H3. We have also examined the selective, modification of chromosomal proteins by different polycyclic aromatic hydrocarbons and it was observed that 7,12-dimethylbenz[a]-anthracene (DMBA), 3-methylcholanthrene (3-MC) and B[a]P showed qualitative similarities in terms of their protein binding. This suggests that stereospecific interactions leading to binding of reactive metabolites of DMBA, B[a]P and 3-MC to chromosomal proteins share common features.
苯并[a]芘（B[a]P）的代谢产物已被证明能高度特异性地修饰染色体蛋白。使用反式-B[a]P二醇环氧化物的（+）和（-）对映体标记分离的细胞核，我们发现这两种化合物修饰组蛋白H2A和H3的能力存在显著差异。（+）对映体修饰组蛋白H2A和H3，而（-）对映体主要修饰组蛋白H2A，对组蛋白H3的亲和力则低得多。我们还研究了不同多环芳烃对染色体蛋白的选择性修饰，观察到7,12-二甲基苯并[a]蒽（DMBA）、3-甲基胆蒽（3-MC）和B[a]P在蛋白质结合方面表现出定性相似性。这表明导致DMBA、B[a]P和3-MC的活性代谢产物与染色体蛋白结合的立体特异性相互作用具有共同特征。
