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人类细胞中染色质与致癌物相互作用的动力学

The dynamics of chromatin carcinogen interactions in the human cell.

作者信息

Kootstra A

出版信息

Nucleic Acids Res. 1986 Dec 22;14(24):9897-909. doi: 10.1093/nar/14.24.9897.

DOI:10.1093/nar/14.24.9897
PMID:3101040
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341342/
Abstract

Human lung epithelioid cells were treated with Benzo (a) pyrene diol epoxide (anti) in order to establish the binding and removal of covalent adducts in chromosomal components. Isolating two different classes of mononucleosomes, it was found that their DNA contained different concentrations of B(a)PDE-DNA adducts, while in both these mononucleosomal preparations only histones H2A and H3 contained detectable amounts of the carcinogen. Further analysis showed that in the intact human cell the carcinogen-DNA adduct distribution is constantly changing as a function of differential accessibility and repair. These results emphasize the dynamics of chromatin-carcinogen modifications.

摘要

用人肺上皮样细胞与苯并（a）芘二醇环氧化物（反式）进行处理，以确定染色体成分中共价加合物的结合与去除情况。分离出两类不同的单核小体后发现，它们的DNA含有不同浓度的苯并（a）芘二醇环氧化物 - DNA加合物，而在这两种单核小体制剂中，只有组蛋白H2A和H3含有可检测量的致癌物。进一步分析表明，在完整的人类细胞中，致癌物 - DNA加合物的分布会随着差异可及性和修复功能而不断变化。这些结果强调了染色质 - 致癌物修饰的动态性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/341342/24351583368b/nar00293-0355-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/341342/24351583368b/nar00293-0355-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/341342/24351583368b/nar00293-0355-a.jpg

相似文献

1
The dynamics of chromatin carcinogen interactions in the human cell.人类细胞中染色质与致癌物相互作用的动力学
Nucleic Acids Res. 1986 Dec 22;14(24):9897-909. doi: 10.1093/nar/14.24.9897.
2
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本文引用的文献

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A low resolution structure for the histone core of the nucleosome.核小体组蛋白核心的低分辨率结构。
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Formation and removal of B[a]P diol epoxide--DNA adducts in human fibroblasts.人成纤维细胞中苯并[a]芘二醇环氧化物-DNA加合物的形成与去除
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Selective modification of nuclear proteins by polycyclic aromatic hydrocarbons and by benzo[a]pyrene diol epoxides.多环芳烃和苯并[a]芘二醇环氧化物对核蛋白的选择性修饰。
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Noncovalent binding of 7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxytetrahydrobenzo[a]pyrene to deoxyribonucleic acid and its catalytic effect on the hydrolysis of the diol epoxide to tetrol.7β,8α-二羟基-9α,10α-环氧四氢苯并[a]芘与脱氧核糖核酸的非共价结合及其对二醇环氧化物水解为四醇的催化作用。
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Excision of benzo[a]pyrene diol epoxide I adducts from nucleosomal DNA of confluent normal human fibroblasts.
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Carcinogen aflatoxin B1 is located preferentially in internucleosomal deoxyribonucleic acid following exposure in vivo in rainbow trout.致癌物黄曲霉毒素B1在虹鳟鱼体内暴露后优先定位在核小体间脱氧核糖核酸中。
Biochemistry. 1980 Dec 9;19(25):5836-42. doi: 10.1021/bi00566a027.