Wong S L, Doi R H
J Biol Chem. 1982 Oct 25;257(20):11932-6.
An analysis of the peptide maps of the sigma factors and core-associated subunits of Bacillus subtilis RNA polymerase has revealed that all the sigma factors ad core-associated polypeptides are derived from separate genes and are not proteolytically modified products of the major 55,000-dalton sigma factor. A comparison of the peptide pattern of the major B. subtilis and Escherichia coli sigma factors revealed limited homology between them. Furthermore, antibody prepared against the 55,000-dalton B. subtilis sigma factor cross-reacted against the E. coli sigma factor, but not against any of the other B. subtilis sigma factors and core-associated polypeptides. These results unambiguously demonstrate the independently derived nature of the B. subtilis RNA polymerase core-associated subunits and the partial relationship between the major sigma factors of B. subtilis and E. coli.
对枯草芽孢杆菌RNA聚合酶的σ因子和核心相关亚基的肽图分析表明，所有的σ因子和核心相关多肽均来自不同的基因，并非主要的55,000道尔顿σ因子的蛋白水解修饰产物。对枯草芽孢杆菌和大肠杆菌主要σ因子的肽图进行比较，发现它们之间存在有限的同源性。此外，针对55,000道尔顿枯草芽孢杆菌σ因子制备的抗体与大肠杆菌σ因子发生交叉反应，但不与其他任何枯草芽孢杆菌σ因子和核心相关多肽发生反应。这些结果明确证明了枯草芽孢杆菌RNA聚合酶核心相关亚基的独立起源性质，以及枯草芽孢杆菌和大肠杆菌主要σ因子之间的部分关系。
