Goldfarb D S, Wong S L, Kudo T, Doi R H
Mol Gen Genet. 1983;191(2):319-25. doi: 10.1007/BF00334833.
A 1,250 base pair Bacillus subtilis chromosomal HindIII restriction fragment (S fragment) has been cloned into the B. subtilis expression-probe plasmid pGR71. The S fragment induces the expression of the pGR71 chloramphenicol resistance gene shortly after the initiation of sporulation. The transcriptional promoter responsible for the expression of this temporally regulated genetic element has been identified and mapped in vitro. This promoter is recognized exclusively by the minor B. subtilis RNA polymerase that contains the 37,000 dalton sigma factor.
一个1250个碱基对的枯草芽孢杆菌染色体HindIII限制性片段（S片段）已被克隆到枯草芽孢杆菌表达探针质粒pGR71中。S片段在芽孢形成开始后不久诱导pGR71氯霉素抗性基因的表达。负责这种时间调控遗传元件表达的转录启动子已在体外被鉴定并定位。该启动子仅被含有37000道尔顿σ因子的次要枯草芽孢杆菌RNA聚合酶识别。
