Wypych J I, Abeyounis C J, Müller U R, Reisman R E
Int Arch Allergy Appl Immunol. 1984;73(1):14-7. doi: 10.1159/000233430.
Optimal concentrations of honeybee, hornet and yellow jacket venoms were used in the radioallergosorbent test (RAST) before and after dialysis. RAST titers in the majority of sera from insect-allergic patients increased significantly with the dialyzed yellow jacket and hornet venom-coupled discs. Of 26 sera which had negative RASTs with crude venom, 20 became positive with dialyzed venom. No change in RAST results was found with crude and dialyzed honeybee venom. The low molecular weight component in the vespid venoms removed by dialysis had no specific activity in RAST inhibition experiments, suggesting nonspecific blocking of binding sites. These results indicate the importance of the appropriate antigen preparation coupled to discs in the RAST.
在透析前后，分别使用了最佳浓度的蜜蜂、黄蜂和黄胡蜂毒液进行放射变应原吸附试验（RAST）。在大多数昆虫过敏患者的血清中，经透析的黄胡蜂和黄蜂毒液偶联盘使RAST滴度显著升高。在26份对粗毒液RAST呈阴性的血清中，20份对透析毒液呈阳性。粗制和透析后的蜜蜂毒液的RAST结果没有变化。透析去除的胡蜂毒液中的低分子量成分在RAST抑制实验中没有特异性活性，提示其对结合位点的非特异性阻断作用。这些结果表明在RAST中与盘偶联的合适抗原制剂的重要性。
