The potential value of silage in detoxifying aflatoxin B1.

Serial concentrations of aflatoxin B1 ranging from 200 to 1500 p.p.b. in 2% lactic acid solution (which is the aimed concentration of lactic acid in fresh silage) were assayed for detoxification. Thin-layer chromatography analysis, revealed a complete transformation of 1 000 p.p.b. of aflatoxin B1 to a new fluorescing compount corresponding to aflatoxin B2a which is referred to as hydroxydihydro-aflatoxin B1 toxicity test on chickens confirmed Ciegler's findings (4). The results confirm that the chemical changes taken place in the silo can detoxify aflatoxin B1 to aflatoxin B2a.