Dési I, Dura G, Szlobodnyik J, Csuka I
J Toxicol Environ Health. 1977 May;2(5):1053-66. doi: 10.1080/15287397709529503.
The effect of pesticides on cell growth and on the cell layer was examined. The pesticides under study had a similar toxicity for the cell suspension and for the cell layer. Vital staining of the cells allowed a more sensitive determination of the cytotoxic concentrations than did the studies on cell morphology without staining. No correlation was found between the cytotoxic concentrations and LD50 values of mammals. The results were compared with those of earlier experiments on rats in which methods of general toxicology and neurotoxicology were applied. Of the eight compounds tested, in six the tissue culture method proved even more sensitive than did the functional neurotoxicological tests. It is suggested that the in vitro method with cultured cells should be used to complement in vivo functional toxicological procedures.
研究了农药对细胞生长和细胞层的影响。所研究的农药对细胞悬液和细胞层具有相似的毒性。与未染色的细胞形态学研究相比，细胞的活体染色能更灵敏地测定细胞毒性浓度。未发现细胞毒性浓度与哺乳动物的半数致死量（LD50）值之间存在相关性。将结果与早期对大鼠进行的实验结果进行了比较，在早期实验中应用了一般毒理学和神经毒理学方法。在所测试的八种化合物中，六种化合物的组织培养方法比功能性神经毒理学测试更灵敏。建议使用培养细胞的体外方法来补充体内功能性毒理学程序。
