Petrov Iu P, Andreeva E V
Tsitologiia. 1982 Jun;24(6):730-6.
A study was made of the effects of trypsin (0.25%) EDTA (0.02%), DMSO (10%) and suboptimal temperatures (16-18 degrees C) on the line L cells of monolayer and suspension cultures by the method of counter-current distribution in two-phase polymer system Dextran-500/poly (ethylene) glycol-6000. It has been shown that these agents cause the increase in number of cells with low partition coefficient, which is most expressed in the population of monolayer subline cells.
采用两相聚合物系统葡聚糖-500/聚乙二醇-6000逆流分配法，研究了胰蛋白酶（0.25%）、乙二胺四乙酸（0.02%）、二甲基亚砜（10%）和亚适宜温度（16 - 18摄氏度）对单层和悬浮培养的L细胞系的影响。结果表明，这些试剂会导致低分配系数细胞数量增加，这在单层亚系细胞群体中表现最为明显。
