Wooley R E, Gilbert J P, Shotts E B
Am J Vet Res. 1981 Nov;42(11):2010-3.
Antibacterial activity against Escherichia coli was obtained with subminimal inhibitory concentrations of oxytetracycline (OTC) and EDTA-tromethamine. Inhibitory effects were not observed using combinations of dimethyl sulfoxide and OTC or dimethyl sulfoxide and EDTA-tromethamine. Neither EDTA-tromethamine nor OTC used alone was capable of the same degree of inhibition. Using a 2-dimensional Microtiter checkerboard technique, the inhibitory activity of these combinations was studied and isobolograms were plotted. A synergistic effect was seen with combinations of OTC and EDTA-tromethamine. Kinetic studies of microbial death, using subminimal inhibitory concentrations of these agents, confirmed these findings.
土霉素（OTC）和乙二胺四乙酸-三羟甲基氨基甲烷（EDTA- tromethamine）的亚最小抑菌浓度对大肠杆菌具有抗菌活性。使用二甲亚砜与OTC或二甲亚砜与EDTA- tromethamine的组合未观察到抑制作用。单独使用EDTA- tromethamine或OTC均不能产生相同程度的抑制作用。使用二维微量滴定棋盘技术研究了这些组合的抑制活性并绘制了等效线图。OTC和EDTA- tromethamine的组合显示出协同作用。使用这些药物的亚最小抑菌浓度对微生物死亡进行的动力学研究证实了这些发现。
