Fletcher M A, Caldwell K E, Latif Z
Vox Sang. 1982;43(2):57-70.
A glycoprotein was isolated from the membrane of the bovine erythrocyte by refluxing the acetone- and ethanol-extracted stroma residue with 75% ethanol. The glycoprotein was purified by phosphocellulose chromatography, ethanol precipitation, lipidsolvent extraction and DEAE chromatography. The glycoprotein appeared to have two serological determinants, both reactive with antibodies present in the sera of patients with infectious mononucleosis. One of the determinants is similar to the Paul-Bunnell heterophile antigen found on sheep erythrocytes. It is dependent on carbohydrate, including sialic acid residues. Another specificity, seemingly not shared by sheep erythrocytes to any great extent, is resistance to neuraminidase and to alkaline borohydride treatment and thus it may be located either on the polypeptide portion of the molecule or on an alkali-stable oligosaccharide. The purified glycoprotein comprises 73% amino acids. Carbohydrate components and their molar ratios were sialic acid (1.0): galactose (1.5): N-acetylglucosamine (1.1): N-acetylgalactosamine (0.5): mannose (0.1).
通过用75%乙醇回流丙酮和乙醇提取的基质残余物，从牛红细胞膜中分离出一种糖蛋白。该糖蛋白通过磷酸纤维素色谱法、乙醇沉淀法、脂溶剂萃取法和DEAE色谱法进行纯化。该糖蛋白似乎有两个血清学决定簇，均与传染性单核细胞增多症患者血清中的抗体发生反应。其中一个决定簇类似于在绵羊红细胞上发现的保罗-邦内尔嗜异性抗原。它依赖于碳水化合物，包括唾液酸残基。另一种特异性在很大程度上似乎不为绵羊红细胞所共有，即对神经氨酸酶和碱性硼氢化钠处理具有抗性，因此它可能位于分子的多肽部分或碱稳定的寡糖上。纯化的糖蛋白含有73%的氨基酸。碳水化合物成分及其摩尔比为唾液酸（1.0）：半乳糖（1.5）：N-乙酰葡糖胺（1.1）：N-乙酰半乳糖胺（0.5）：甘露糖（0.1）。
