Caldwell K E, Cayer M, Whitney P L, Fletcher M A
Mol Immunol. 1982 Jun;19(6):779-91. doi: 10.1016/0161-5890(82)90004-9.
A highly purified preparation of horse erythrocyte glycoprotein was prepared from an aqueous ethanolic extract of hemoglobin-free membranes. The subunit apparent mol. wt was 30,000. In aqueous solution the glycoprotein formed globular aggregates of 93 +/- 16 A diameter. The glycoprotein had a receptor for the Paul-Bunnell antibody of infectious mononucleosis which was associated with an O-glycosidically linked oligosaccharide and dependent on the presence of N-glycolylneuraminic acid. In addition the glycoprotein had a neuraminidase-sensitive receptor for human peripheral blood lymphocytes. Fifty per cent inhibition of the rosetting of sheep red cells by 4 x 10(5) lymphocytes was caused by 30 microgram of glycoprotein.
从无血红蛋白膜的乙醇水溶液提取物中制备了高度纯化的马红细胞糖蛋白制剂。亚基的表观分子量为30,000。在水溶液中，糖蛋白形成直径为93±16 Å的球状聚集体。该糖蛋白具有针对传染性单核细胞增多症的保罗-邦内尔抗体的受体，该受体与O-糖苷键连接的寡糖相关，并依赖于N-羟乙酰神经氨酸的存在。此外，该糖蛋白具有对人外周血淋巴细胞敏感的神经氨酸酶受体。30微克糖蛋白可导致4×10⁵个淋巴细胞对绵羊红细胞玫瑰花结形成的抑制率达到50%。
