通过标记拯救法检测禽白血病病毒感染细胞的非感染性DNA片段
Assay of noninfectious fragments of DNA of avian leukosis virus-infected cells by marker rescue.
作者信息
Cooper G M, Castellot S B
出版信息
J Virol. 1977 May;22(2):300-7. doi: 10.1128/JVI.22.2.300-307.1977.
A marker rescue assay of noninfectious fragments of avian leukosis virus DNAs is describe. DNA fragments were prepared either by sonication of EcoRI-digestion of DNAs of chicken cells infected with wild-type Rous sarcoma virus, with a nontransforming avian leukosis virus, and with a mutant of Rous sarcoma virus temperature sensitive for transformation. Recipient cultures of chicken embryo fibroblasts were treated with noninfectious DNA fragments and infected with temperature-sensitive mutants of Rous sarcoma virus defective in DNA polymerase or in an internal virion structural protein. Wild-type progeny viruses which replicated at the nonpermissive temperature were isolated. Some of the wild-type progeny acquired both the wild-type DNA polymerase and the subgroup specificity of the Rous sarcona virus strain used for preparation of sonicated or EcoRI-digested DNA fragments. Therefore the genetic markers for DNA polymerase and envelope were linked and appeared to be located on the same EcoRi fragment of the DNA of Rous sarcoma virus-infected cells.
本文描述了禽白血病病毒DNA非感染性片段的标记拯救试验。DNA片段通过对感染野生型劳氏肉瘤病毒、非转化性禽白血病病毒以及对转化温度敏感的劳氏肉瘤病毒突变体的鸡细胞DNA进行EcoRI酶切后超声处理制备。用非感染性DNA片段处理鸡胚成纤维细胞受体培养物，并感染DNA聚合酶或内部病毒体结构蛋白有缺陷的劳氏肉瘤病毒温度敏感突变体。分离出在非允许温度下复制的野生型子代病毒。一些野生型子代同时获得了野生型DNA聚合酶和用于制备超声处理或EcoRI酶切DNA片段的劳氏肉瘤病毒株的亚群特异性。因此，DNA聚合酶和包膜的遗传标记是连锁的，并且似乎位于感染劳氏肉瘤病毒的细胞DNA的同一条EcoRi片段上。
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