与编码RNA指导的DNA聚合酶的禽白血病病毒基因相关的内源性细胞遗传信息的标记拯救。
Marker rescue of endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase.
作者信息
Cooper G M
出版信息
J Virol. 1978 Mar;25(3):788-96. doi: 10.1128/JVI.25.3.788-796.1978.
Abstract
Endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase was studied, using a marker rescue assay to detect biological activity of subgenomic fragments of virus-related DNAs of uninfected avian cells. Recipient cultures of chicken embryo fibroblasts were treated with sonicated DNA fragments and were infected with a temperature-sensitive mutant of Rous sarcoma virus that encoded a thermolabile DNA polymerase. Wild-type progeny viruses were isolated by marker rescue with fragments of DNA of uninfected chicken, pheasant, quail, and turkey cells. The DNAs of these uninfected avian cells, therefore, appeared to contain endogenous genetic information related to the avian leukosis virus DNA polymerase gene.
摘要
利用一种标记拯救试验来检测未感染禽类细胞中病毒相关DNA亚基因组片段的生物活性，对与编码RNA指导的DNA聚合酶的禽白血病病毒基因相关的内源性细胞遗传信息进行了研究。用超声破碎的DNA片段处理鸡胚成纤维细胞的受体培养物，并用编码热不稳定DNA聚合酶的劳氏肉瘤病毒温度敏感突变体进行感染。通过用未感染的鸡、雉鸡、鹌鹑和火鸡细胞的DNA片段进行标记拯救，分离出野生型子代病毒。因此，这些未感染禽类细胞的DNA似乎含有与禽白血病病毒DNA聚合酶基因相关的内源性遗传信息。
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