利血平在非质子有机溶剂体系中的微分脉冲和交流极谱法。

Differential pulse and AC polarography of reserpine, using an aprotic organic solvent system.

作者信息

Taira A, Smith D E

出版信息

J Assoc Off Anal Chem. 1978 Jul;61(4):941-8.

Abstract

Previously demonstrated advantages of aprotic organic solvent for analysis of organic pharmaceuticals have been applied to the assay of reserpine tablets. A procedure using high sensitivity electroanalytical relaxation methods was developed which is simpler and more sensitive than the USP assay. Most data were obtained with a commercial differential pulse polarograph which is widely available. However, less extensive measurements with other instruments established the general applicability of the differential pulse method, as well as various acpolarographic techniques. The results of differential pulse polarographic (DPP) and USP assays of synthetically formulated reserpine tablets compare favorably. The DPP method yields an average recovery for 0.1 mg reserpine/tablet of 100%, with a range of 97.6-102.3%. Corresponding data for 0.25 mg reserpine/tablet are 99.3%, with a range of 97.6-100.6%. Satisfactory results also were obtained in content uniformity studies, using single 0.1 and 0.25 mg tablets.

摘要

先前已证明的非质子有机溶剂在有机药物分析中的优势已应用于利血平片的测定。开发了一种使用高灵敏度电分析弛豫方法的程序，该程序比重试药品标准中规定的测定方法更简单、更灵敏。大多数数据是使用广泛可用的商用微分脉冲极谱仪获得的。然而，使用其他仪器进行的较少范围的测量确定了微分脉冲法以及各种交流极谱技术的普遍适用性。合成配制的利血平片的微分脉冲极谱法（DPP）和美国药典测定结果相比很有利。DPP 法对每片含 0.1 mg 利血平的平均回收率为 100%，范围为 97.6 - 102.3%。每片含 0.25 mg 利血平的相应数据为 99.3%，范围为 97.6 - 100.6%。在含量均匀度研究中，使用单片 0.1 mg 和 0.25 mg 的片剂也获得了满意的结果。