Cantwell S G, Lau E P, Watt D S, Fall R R
J Bacteriol. 1978 Aug;135(2):324-33. doi: 10.1128/jb.135.2.324-333.1978.
The ability of various pseudomonads to utilize acyclic isoprenoids as a sole carbon source was investigated. Tests for utilization of acyclic isoprenols such as citronellol and geraniol were complicated by toxic effects of these alcohols, and most species tested were killed by exposure to citronellol or geraniol (0.1%, vol/vol) in liquid culture. In the case of Pseudomonas citronellolis, sensitivity to isoprenols is reduced by prior induction of the isoprenoid degradative pathway via either growth on succinate in the presence of citronellol or growth on citronellic acid. For this species, citronellic acid proved to be the best isoprenoid growth substrate tested. Geraniol utilization as a taxonomic indicator for different subgroups of pseudomonads is discussed. Only a few of the species tested were able to utilize acyclic isoprenoids. Two species which utilize C10 acyclic isoprenoids, P. aeruginosa and P. mendocina, were shown to contain the inducible enzyme geranyl-coenzyme A carboxylase, one of the unique enzymes in the isoprenol degradative pathway known to occur in P. citronellolis. Of the species which utilized geranitol, none showed definite growth on the homologous C15 and C20 isoprenols.
研究了各种假单胞菌利用无环类异戊二烯作为唯一碳源的能力。对香茅醇和香叶醇等无环异戊烯醇的利用测试因这些醇的毒性作用而变得复杂，大多数测试菌株在液体培养中暴露于香茅醇或香叶醇（0.1%，体积/体积）时会死亡。对于香茅假单胞菌而言，通过在香茅醇存在下以琥珀酸盐为碳源生长或在香茅酸上生长来预先诱导类异戊二烯降解途径，可降低其对异戊烯醇的敏感性。对于该菌株，香茅酸被证明是所测试的最佳类异戊二烯生长底物。讨论了将香叶醇利用作为假单胞菌不同亚组的分类指标。在所测试的菌株中，只有少数能够利用无环类异戊二烯。两种能够利用C10无环类异戊二烯的菌株，铜绿假单胞菌和门多萨假单胞菌，被证明含有可诱导的香叶基辅酶A羧化酶，这是香茅醇降解途径中已知存在于香茅假单胞菌中的独特酶之一。在利用香叶醇的菌株中，没有一种在同源的C15和C20异戊烯醇上表现出明显的生长。
