Katoh Y, Takayama S
Carcinogenesis. 1982;3(8):867-73. doi: 10.1093/carcin/3.8.867.
The growth responses of hamster dermal fibroblasts (HDF cells) to platelet-derived growth factor (PDGF) during chemical carcinogenesis in vitro were investigated. Normal HDF cell grew in medium with whole blood serum (WBS), but not in medium with plasma-derived serum (PDS). However, they grew in PDS medium when PDGF was added. In contrast to control cultures which finally stopped proliferating, 7 out of 8 cell strains treated with 4-nitroquinoline-1-oxide (4NQO) or N-methyl-N'-nitro-nitrosoguanidine changed to cell lines. Later, 5 of these 7 cell lines became able to grow in PDS medium in association with ability to grow in soft agar. When clonal cell lines were isolated at early stages of carcinogenesis while parental cell lines were still PDGF-dependent, most of them gradually became PDGF-independent as well. Dialysed cell lysates of transformed HDF cells showed strong growth stimulating activity on normal HDF cells in PDS medium. Thus, this conversion of PDGF-dependent cells to PDGF-independent cells was correlated with the appearance of a growth factor(s) produced specifically by transformed HDF cells.
研究了仓鼠皮肤成纤维细胞（HDF细胞）在体外化学致癌过程中对血小板衍生生长因子（PDGF）的生长反应。正常HDF细胞在含全血血清（WBS）的培养基中生长，但在含血浆衍生血清（PDS）的培养基中不生长。然而，当添加PDGF时，它们在PDS培养基中生长。与最终停止增殖的对照培养物相反，用4-硝基喹啉-1-氧化物（4NQO）或N-甲基-N'-硝基-N-亚硝基胍处理的8个细胞株中有7个转变为细胞系。后来，这7个细胞系中的5个变得能够在PDS培养基中生长，并具有在软琼脂中生长的能力。当在致癌早期分离克隆细胞系时，亲本细胞系仍依赖PDGF，其中大多数也逐渐变得不依赖PDGF。转化的HDF细胞的透析细胞裂解物对PDS培养基中的正常HDF细胞显示出强烈的生长刺激活性。因此，这种从依赖PDGF的细胞向不依赖PDGF的细胞的转变与转化的HDF细胞特异性产生的一种生长因子的出现相关。
