Katoh Y
J Cancer Res Clin Oncol. 1985;110(2):177-80. doi: 10.1007/BF00402736.
Cell lysates from transformed hamster dermal fibroblasts (HDF cells) induced colony formation of normal HDF cells in soft agar medium and also stimulated proliferation of normal HDF cells in medium supplemented with plasma-derived serum (PDS). The colony forming activities of cell lysates correlated well with their growth stimulating activities in PDS-supplemented medium. The growth factor(s) were shown to be sensitive to trypsin and dithiothreitol (DTT), but heat stable. However, these transformed HDF cell lines did not all produce tumor nodules when injected into hamsters. This was not due to immunological rejection because similar results were obtained in hamsters treated with anti-hamster thymocyte serum (ATS). Thus it was concluded that production of these growth factor(s) by transformed HDF cell lines is not itself sufficient for acquisition of complete malignancy in vivo.
来自转化的仓鼠真皮成纤维细胞（HDF细胞）的细胞裂解物在软琼脂培养基中诱导正常HDF细胞形成集落，并且在补充有血浆来源血清（PDS）的培养基中刺激正常HDF细胞的增殖。细胞裂解物的集落形成活性与其在补充PDS的培养基中的生长刺激活性密切相关。生长因子对胰蛋白酶和二硫苏糖醇（DTT）敏感，但对热稳定。然而，当将这些转化的HDF细胞系注射到仓鼠体内时，并非所有细胞系都产生肿瘤结节。这不是由于免疫排斥，因为在用抗仓鼠胸腺细胞血清（ATS）处理的仓鼠中获得了相似的结果。因此得出结论，转化的HDF细胞系产生这些生长因子本身不足以在体内获得完全的恶性肿瘤特性。
