Li X, Vogt W
Immunopharmacology. 1982 Oct;5(1):31-8. doi: 10.1016/0162-3109(82)90034-0.
The effects of an immunostimulating polysaccharide, Bo, from sugar cane, on the complement system have been investigated. Bo, a glucan of about 10,000 mol wt, was found to activate complement in whole human and guinea pig serum in vitro by the classical pathway. Complement consumption was also demonstrated in guinea pigs upon intravenous injection. Specifically, C1 is activated, and C4 and C2, as well as C3, are consumed. The activation is prevented when Ca++ ions are chelated by ethyleneglycoltetraacetic acid, and when C1q is lacking. Hence, it does not rest on direct activation of C1s. Supplementation of C1q-deficient human serum with purified C1q restores the ability to be activated by Bo. The alternative pathway of complement is little if at all affected by the polysaccharide. The activation of C1 seems to be mediated by immune complex formation between Bo and naturally occurring immunoglobulins. Complement in sera from two severely hypogammaglobulinemic patients was not activated by Bo, but was made reactive by addition of purified human immunoglobulin G.
对一种来自甘蔗的免疫刺激多糖Bo对补体系统的作用进行了研究。Bo是一种分子量约为10,000的葡聚糖，发现在体外能通过经典途径激活正常人血清和豚鼠血清中的补体。静脉注射Bo后，在豚鼠体内也证实了补体的消耗。具体而言，C1被激活，C4、C2以及C3被消耗。当钙离子被乙二醇四乙酸螯合时，以及当缺乏C1q时，激活作用被阻止。因此，它并非基于C1s的直接激活。用纯化的C1q补充C1q缺陷的人血清可恢复被Bo激活的能力。补体的替代途径几乎不受该多糖的影响。C1的激活似乎是由Bo与天然存在的免疫球蛋白之间形成免疫复合物介导的。两名严重低丙种球蛋白血症患者血清中的补体未被Bo激活，但加入纯化的人免疫球蛋白G后变得有反应性。
