Levi H, Nielsen A
J Invest Dermatol. 1982 Nov;79(5):292-6. doi: 10.1111/1523-1747.ep12500080.
Under normal conditions, the toad Bufo bufo bufo molts about once a week. It was considered of interest to study whether the rhythmic pattern of cell loss through shedding of a stratum corneum is correlated with a rhythmic production of cells in stratum germinativum. An autoradiographic investigation of cell kinetics in toad skin using [3H]-thymidine and [14C]-thymidine as precursors revealed that cell production in stratum germinativum is a continuous process, and the cell production rate is much higher than is required for replacement of the cells lost with each molt. The parameters measured, i.e., % labeled cells, length of cell cycle, and S-phase, as well as migration of labeled cells from stratum germinativum toward stratum corneum support the assumption of an inhomogeneous cell population in stratum germinativum. A model proposed by Potten form the cellular organization of mouse epithelium provides a useful basis for the interpretation of the results obtained in the present study.
在正常情况下，蟾蜍中华大蟾蜍大约每周蜕皮一次。研究角质层脱落导致的细胞丢失的节律模式是否与生发层细胞的节律性产生相关，被认为是很有意义的。使用[3H]-胸腺嘧啶核苷和[14C]-胸腺嘧啶核苷作为前体对蟾蜍皮肤细胞动力学进行的放射自显影研究表明，生发层中的细胞产生是一个连续的过程，并且细胞产生速率远高于每次蜕皮所丢失细胞的替换所需速率。所测量的参数，即标记细胞百分比、细胞周期长度和S期，以及标记细胞从生发层向角质层的迁移，支持了生发层中细胞群体不均匀的假设。Potten提出的小鼠上皮细胞组织模型为解释本研究获得的结果提供了有用的基础。
