Stowell C P, Lee Y C
J Biol Chem. 1978 Sep 10;253(17):6107-10.
The binding of D-glucosyl-neoglycoproteins and D-galactose-terminated glycoproteins to the hepatic asialoglycoprotein receptor of rabbit liver membranes were characterized and compared. The binding of both types of glycoproteins showed the same dependence on calcium concentration, sensitivity to neuraminidase, and degree of inhibition by various carbohydrate derivatives. These results, along with the observation that the rabbit liver membranes bound both the D-glucosyl- and D-galactosyl-terminated glycoproteins to the same extent, indicated that both types of glycoproteins bound to the same receptor. To confirm this hypothesis, receptors were isolated from rabbit livers by affinity chromatography using D-galactosyl-bovine serum albumin or D-glucosyl-bovine serum albumin immobilized on Sepharose. These receptors were shown to be identical by several chemical and immunological criteria as well as in their ability to bind equal amounts of D-galactosyl- and D-glucosyl-terminated glycoproteins. The conclusion is that the rabbit hepatic asialoglycoprotein receptor cannot discriminate between D-galactosyl and D-glucosyl-terminated glycoproteins and binds both.
对D-葡糖基-新糖蛋白和D-半乳糖末端糖蛋白与兔肝细胞膜肝去唾液酸糖蛋白受体的结合进行了表征和比较。两种类型糖蛋白的结合对钙浓度的依赖性相同，对神经氨酸酶敏感，并且受到各种碳水化合物衍生物的抑制程度相同。这些结果，连同兔肝细胞膜以相同程度结合D-葡糖基末端和D-半乳糖基末端糖蛋白的观察结果，表明两种类型的糖蛋白都与同一受体结合。为了证实这一假设，使用固定在琼脂糖上的D-半乳糖基-牛血清白蛋白或D-葡糖基-牛血清白蛋白通过亲和色谱法从兔肝脏中分离受体。通过几种化学和免疫学标准以及它们结合等量D-半乳糖基末端和D-葡糖基末端糖蛋白的能力表明这些受体是相同的。结论是兔肝去唾液酸糖蛋白受体不能区分D-半乳糖基和D-葡糖基末端糖蛋白，并且两者都能结合。
