Salmeron G, Lipsky P E
J Rheumatol Suppl. 1982 Jul-Aug;8:25-31.
The effect of auranofin (AF) on in vitro correlates of human immune responsiveness was examined. AF inhibited mitogen induced human lymphocyte proliferation and the generation of immunoglobulin secreting cells in a concentration dependent manner. The inhibition was most effective when AF was present from the initiation of culture indicating that this drug blocked a critical early step in lymphocyte activation. Marked inhibition of mitogen responsiveness was observed as a result of a 1-h preincubation with AF. The brief preincubation with low concentrations of AF (0.3 micrograms/ml) resulted in a selective inhibition of the accessory function of monocytes but had no effect on potential lymphocyte responsiveness. Preincubation with higher concentrations of AF (greater than 0.6 micrograms/ml) resulted in a more non-specific inhibition of both monocyte and lymphocyte function. These data support the conclusion that AF may function as an immunosuppressive agent.
研究了金诺芬（AF）对人类免疫反应性体外相关指标的影响。AF以浓度依赖性方式抑制有丝分裂原诱导的人类淋巴细胞增殖和免疫球蛋白分泌细胞的产生。当从培养开始就存在AF时，抑制作用最为有效，这表明该药物阻断了淋巴细胞活化过程中的关键早期步骤。与AF预孵育1小时后，观察到有丝分裂原反应性受到明显抑制。用低浓度AF（0.3微克/毫升）进行短暂预孵育导致单核细胞辅助功能的选择性抑制，但对潜在的淋巴细胞反应性没有影响。用较高浓度AF（大于0.6微克/毫升）进行预孵育导致单核细胞和淋巴细胞功能更非特异性的抑制。这些数据支持AF可能作为一种免疫抑制剂发挥作用的结论。
