Fischer O
Vet Med (Praha). 1982 Aug;27(8):465-71.
It has been found out by examining faeces of 83 calves from calf house in Uhercice (Breclav district) that 52 calves excreted coccidium oocysts, genus Cryptosporidium Tyzzer, 1907, during the period of milk nutrition. The calves excreted oocysts from the eighth day of age, and at the end of the milk nutrition period (43rd-47th ridium Tyzzer, 1907, during the period of milk nutrition. The calves excreted oocysts of cryptosporidia were found in two thirds of calves suffering from diarrheas. By histological examination of a 27-day-old calf, developmental stages of this coccidium were found on the surface of villus epithelium in the distal part of ileum. Villi were shortened and expanded, submucous connective tissue was penetrated by inflammatory infiltrate with large quantity of eosinophil granulocytes. For demonstration of oocysts in faeces, Breza flotation method combined with sedimentation and staining by modified Giemsa panoptic method were used.
通过对乌赫尔采（布雷clav 区）犊牛舍的 83 头犊牛粪便进行检查发现，52 头犊牛在哺乳期间排出了隐孢子虫属（Cryptosporidium Tyzzer，1907）的球虫卵囊。犊牛从 8 日龄开始排出卵囊，在哺乳期末期（第 43 - 47 天）排出。在三分之二腹泻的犊牛中发现了隐孢子虫卵囊。通过对一头 27 日龄犊牛进行组织学检查，在回肠远端绒毛上皮表面发现了这种球虫的发育阶段。绒毛缩短并扩张，黏膜下结缔组织被大量嗜酸性粒细胞浸润的炎性细胞穿透。为了在粪便中检测卵囊，采用了布雷扎浮选法结合沉淀法，并通过改良吉姆萨全景染色法进行染色。
