[通过酶联免疫吸附测定法检测新城疫病毒]
[Demonstration of Newcastle disease virus by enzyme-linked immunosorbent assay].
作者信息
Dumanova L, Vasileva L, Chenchev I
出版信息
Vet Med Nauki. 1982;19(3):46-51.
Investigations were carried out with regard to the application of the sensitive, rapid, and effective enzyme-linked immunosorbent assay (ELISA) in the demonstration of the Newcastle disease virus (NDV). Used was the peroxydase-marked immunoglobulin fraction of the anti-DV rabbit serum in the microconditions of the double-antibody method. The demonstration of the NDV was in a cultivation liquid of NDV-infected BHK21 cells. As control served intact cells as well as strains Roussev and La Sota of NDV pruified through ultracentrifugation on an isokinetic saccharose gradiend. It is stated that ELISA is much more sensitive than the hemagglutination test and the agar gel immunodiffusion test.
针对灵敏、快速且有效的酶联免疫吸附测定法（ELISA）在新城疫病毒（NDV）检测中的应用展开了研究。在双抗体法的微量条件下，使用了抗新城疫病毒兔血清的过氧化物酶标记免疫球蛋白组分。新城疫病毒的检测是在感染新城疫病毒的BHK21细胞培养液中进行的。作为对照的是未感染的细胞以及通过等速蔗糖梯度超速离心纯化的新城疫病毒Roussev株和La Sota株。结果表明，ELISA比血凝试验和琼脂凝胶免疫扩散试验灵敏得多。
Zotero 插件