Williams R, Boshoff C H, Verwoerd D, Schoeman M, van Wyk A, Gerdes T H, Roos K
Onderstepoort Veterinary Institute, Republic of South Africa.
Avian Dis. 1997 Oct-Dec;41(4):864-9.
A two-graph receiver operating characteristic analysis, performed on the hemagglutination-inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) test results of a Newcastle disease virus (NDV)-positive and NDV-negative control group of ostrich sera, proved that the ELISA was superior to the HI in both sensitivity and specificity. Comparison of results of the two assays performed on a panel of simulated positive sera ranging from very weak to very strong showed that the ELISA was at least 10 times more sensitive than the HI in detecting low levels of ostrich antibodies to NDV. The ELISA also has the advantage of using untreated serum in a single dilution as opposed to the HI test, which uses pretreated serum in titration.
对一组新城疫病毒（NDV）阳性和阴性鸵鸟血清对照组的血凝抑制（HI）试验及酶联免疫吸附测定（ELISA）试验结果进行双图接收器操作特征分析，结果表明ELISA在敏感性和特异性方面均优于HI。对一组从非常弱到非常强的模拟阳性血清进行的两种检测方法结果比较显示，在检测低水平鸵鸟抗新城疫病毒抗体时，ELISA的敏感性至少是HI的10倍。与HI试验（使用经预处理的血清进行滴定）不同，ELISA的优点是使用未经处理的单一稀释血清。
