Pyant K S, Brierley G P
Experientia. 1982 Oct 15;38(10):1202-4. doi: 10.1007/BF01959740.
In the absence of divalent cations, ionophore A23187 supports low rates of monovalent cations loss (Na+ greater than K+) from unilamellar liposomes containing the sulfate salts. Monovalent cation efflux is optimal when a pH gradient (interior alkaline) is applied. The maximum observed rate of 0.56 ngion K+ . min-1 . nmol-1 A23187 is insufficient to account for the rates of K+ efflux induced by the ionophore in mitochondria (150 ngion K+ . min-1 . nmol-1 A23187). These studies therefore support the concept that A23187 induces loss of K+ from mitochondria by removal of regulating divalent cations from an endogenous K+/H+ exchanger.
在没有二价阳离子的情况下，离子载体A23187能使含有硫酸盐的单层脂质体以低速率丢失单价阳离子（Na⁺丢失速率大于K⁺）。当施加pH梯度（内部呈碱性）时，单价阳离子外流达到最佳状态。观察到的最大速率为0.56纳摩尔K⁺·分钟⁻¹·纳摩尔⁻¹ A23187，这一速率不足以解释该离子载体在线粒体中诱导的K⁺外流速率（150纳摩尔K⁺·分钟⁻¹·纳摩尔⁻¹ A23187）。因此，这些研究支持了这样一种观点，即A23187通过从内源性K⁺/H⁺交换体中去除调节性二价阳离子，从而诱导线粒体中K⁺的丢失。
